銦對植物的毒性及土壤銦對食用米麥的環境風險
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詳目顯示 ; 銦對植物的毒性及土壤銦對食用米麥的環境風險 · Indium toxicity to plants and environmental risk of indium contaminated soils for growing rice and wheat.
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本論文永久網址: 複製永久網址Twitter研究生:張馨方研究生(外文):Hsin-FangChang論文名稱:銦對植物的毒性及土壤銦對食用米麥的環境風險論文名稱(外文):Indiumtoxicitytoplantsandenvironmentalriskofindiumcontaminatedsoilsforgrowingriceandwheat指導教授:王尚禮、葉國楨指導教授(外文):Shan-LiWang、Kuo-ChenYeh口試委員:李達源、莊愷瑋、鄒裕民、劉雨庭、賴鴻裕、楊淑怡口試委員(外文):Dar-YuanLee、Kai-WeiJuang、Yu-MinTzou、Yu-TingLiu、Hung-YuLai、Shu-YiYang口試日期:2020-07-29學位類別:博士校院名稱:國立臺灣大學系所名稱:農業化學研究所學門:農業科學學門學類:農業化學類論文種類:學術論文論文出版年:2020畢業學年度:108語文別:英文論文頁數:131中文關鍵詞:銦、阿拉伯芥、水稻、小麥、逆境反應、植物營養、健康風險外文關鍵詞:Indium、Arabidopsisthaliana、Rice、Wheat、Stressresponse、Nutrientuptake、HealthriskDOI:10.6342/NTU202003574相關次數:
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銦之化合物被廣泛的使用在半導體、光電和能源等高科技產品當中,隨著使用量的增加,也增加了曝露於環境中的風險,若此污染物流布於土壤當中,則可能累積於植物體內,進入食物鏈進一步影響人體的健康。
因此為評估其污染風險,有必要瞭解銦進入土壤後的生物有效性,以及食用作物對其吸收和累積。
銦在土壤中主要是鐵猛氧化物結合態的形式存在,其次是氫氧化銦和磷酸銦的沈澱。
銦化合物的溶解度決定了土壤中銦的有效性,在pH低時,銦的有效性較高。
植物吸收的銦大部分會積累在根部,只有一小部分被運送至穀粒。
銦的積累會限制植物的生長、引起氧化逆境、花青累積與植體內營養的失衡。
植物根系分泌物中的檸檬酸可以作為一個潛在銦的螯合劑,降低銦的吸收。
食用受銦污染的糧食作物可能是人類暴露銦污染的接觸途徑之一。
為了評估食用銦污染米麥對人體健康風險造成危害程度,引用美國環保署環境風險評估公式運算銦之危害商數值。
水稻和小麥分別種植在不同pH的土壤中,其中高銦濃度(1.0mmolkg-1)的酸性土壤,被認為是現實中最壞的污染情況,因為銦在酸性條件下有最大的生物有效性。
結果顯示即使在此條件下,從受銦污染的土壤中收獲的米麥,銦的最大危害商數值皆小於1,即表示不會對人類健康產生不利影響,依此,可推測銦於食用米麥的食物鏈途徑對人體的危害風險應該不高。
然而,實地銦污染如何藉由食物鏈影響人體的健康仍須進一步研究,以更好地闡明種植在受銦污染土壤中的作物所帶來的環境風險。
Theuseofindiuminsemiconductorproductshasincreasedmarkedlyinrecentyears.Thereleaseofindiumintotheecosystemisinevitable.Undersuchcircumstances,effectiveandaccurateassessmentofindiumriskisimportant.However,knowledgeofitsenvironmentalfatehasbeenverylimitedsofar.Thisstudyaimstoinvestigateindiumaccumulationanditstoxiceffectsonplants,asplantsconstitutethebaseoffoodchains.Insoil,thepredominantindiumspeciesareindiumassociatedwithironhydroxidesfollowedbyindiumhydroxideandphosphateprecipitates.Theirsolubilitiesdetermineoverallindiumavailabilityinsoil,whichishigheratlowerpHs.Mostabsorbedindiumaccumulatesattheroots,withonlyatinyportionreachingthegrains.Indiumaccumulationinplantscausedstuntedgrowth,oxidativestress,anthocyanization,andunbalancedmineralnutrition.Secretedcitratecouldfunctionasapotentialdetoxifiertorepelindiumuptake.Consumptionofindium-contaminatedfoodcropsispotentiallyamajorhumanexposurepathway.Indiumaccumulationinthehumanbodyisprobablycarcinogenicandhasvariousadverseeffectsonhumanhealth,suchasheart,kidney,andliverdamages.Toevaluatepotentialrisksassociatedwithindium-contaminatedconsumption,riceandwheatweregrownonsoilswithdifferentpHsandtextures,includingacidicsoilwithahighindiumconcentration(1.0mmolkg-1),whichisconsideredtheworst-casescenariobecauseitpromotesthegreatestindiumbioavailability.Thehealthriskstohumans,basedontheirgrainconsumption,wereevaluatedusingtheHazardQuotient(HQ).ThecorrespondingHQindicatednoadverseeffectsonhumanhealth,evenintheworst-casescenario.Therefore,theconsumptionofriceandwheatgrainsharvestedfromindium-contaminatedsoilsprobablydoesnotwarrantconcernforhumanhealth.Furtherfieldstudiesarenecessarytobetterelucidatetherisksassociatedwithconsumingcropsgrowninindiumcontaminatedsoils.
AbstractiTableofcontentsiiiListoftablesviListoffiguresviiChapter1.GeneralIntroduction1Chapter2.Literaturereview52.1Thechronicleofindium62.1.1Physicalandchemicalpropertiesofindiumanditsapplication62.2Environmentalaspects72.2.1Indiumasanenvironmentalcontaminant72.2.2Transportanddistributionofindiuminair,waterandsoil82.2.3Environmentalstandardsforindium112.3Indiumanditstoxiceffects132.3.1Exposuredatainexperimentalanimals132.3.2Carcinogenicity,Mutagenicity,andTeratogenicity142.3.3Healthrisksofindiuminhumans142.3.4Effectsofindiumonplantgrowth15Chapter3.Indiumaccumulation,toxicity,andtoleranceinthemodalplantArabidopsisthaliana163.1Introduction173.2Materialandmethods183.2.1IndiumTreatmentsandPlantGrowth183.2.2ElementalAnalysis183.2.3LipidPeroxidationEstimation183.2.4AnthocyaninProfilingAnalysis193.2.5RNAIsolationandQuantitativeReal-timePCR193.2.6Proteinextractionandimmunoblotanalysis203.2.7NanoSIMSAnalysis213.2.8LA-ICP-MSAnalysis223.2.9IndiumK-edgeXASAnalysis223.2.10RootExudatesAnalysis243.2.11Statisticalanalysis243.3Resultsanddiscussion253.3.1IndiumaccumulationanditseffectonthegrowthofArabidopsis253.3.2Oxidativestressandantioxidativesysteminindium-treatedArabidopsis273.3.3EffectofindiumonmineralcontentinArabidopsis323.3.4Genesofphosphatestarvationresponseareinducedbyindiumexposure373.3.5IndiumtreatmentreducesproteinlevelsoftransportersinPiuptakeandroot-to-shoottranslocation393.3.6Indium-stimulatedPHO1degradationisviaPHO2433.3.7Impairedphosphorushomeostasisisakeydeterminantofindiumtoxicity453.3.8SpatialdistributionandspeciationofindiuminArabidopsis493.3.9Indiuminducessecretionofrootorganicacids563.4EnvironmentalImplications59Chapter4.Indiumuptakeandaccumulationbyriceandwheat,itseffectsonplantsnutrients,andassociatedhealthriskviacrop’sgrainsconsumption614.1Introduction624.2Materialandmethods644.2.1Soilpreparation644.2.2Potexperiments654.2.3ElementalAnalysis664.2.4Determinationofthebioaccumulationfactor674.2.5IndiumK-edgeXASanalysis674.2.6Sequentialextraction684.2.7Micro-XRFandindiumK-edgeMicro-XANESanalysis694.2.8LaserablationICP-MS(LA-ICP-MS)analysis694.2.9Humanriskassessmentofindiumthroughriceorwheatconsumption704.2.10Statisticalanalysis714.3Resultsanddiscussion724.3.1Indiumuptakeofriceandwheatplantsasafunctionofsoilindiumcontent724.3.2Indiumaccumulationindifferentpartsofriceandwheatplants764.3.2.1Indiumavailabilityinsoils764.3.2.2Distributionandspeciationofindiuminthesoilsusingμ-XRFandμ-XANES834.3.2.3Bioaccumulationfactorsofindiuminriceandwheat884.3.3Risksofriceandwheatgrainconsumptiontohumanhealth914.3.4Effectsofindiumonplantsnutrients934.4EnvironmentalImplications94Chapter5.Concludingremarksandfutureperspectives965.1Introduction975.2Bioavailabilityofindiuminsoil985.3Indiumuptake,transportanddistributioninplants1035.4Generaleffectsofindiumonplants1065.4.1Indium-inducedoxidativestress1065.4.2Antioxidantresponsesinplantstoindium-inducedoxidativestress1075.4.3Nutrientuptakeunderindiumstress1075.4.5Mechanismofindiumtoleranceinplants:thepossibleroleoforganicacids1095.4.6Aluminum,galliumandindiumtoxicityinplants1115.5Indiumaccumulationinstaplecrops,amajorexposurepathwayforindiumpollutioninenvironment,doesnotposeahealthrisk1135.6Conclusions115References116AppendixI.Supportinginformation129AppendixII.StatementofContribution131
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