洪金俊– 中央研究院生物化學研究所

洪金俊博士. 研究副技師. 中央研究院生物化學研究所406室. Skiptocontent 洪金俊博士 研究副技師 中央研究院生物化學研究所406室 研究領域與專長 ProvidingtrainingandtechnicalexpertisetoIBCinvestigatorsinterestedinusingmicroscopy,flowcytometerandscientificimagingintheirresearch Modalitiesandtechniquesincludinglaserscanningconfocalmicroscopy,wide-fieldfluorescencemicroscopy,livecellimaging Indepthcollaborationonselectprojectsinvolvingadvancedmicroscopytechniquesandmethodologies Maintenanceoftheinstrumentationandassociatedinfrastructure AssistancewitheducationalmicroscopycoursesconductedatIBC 學經歷 經歷 2014– 迄今 研究副技師,中研院生化所 2009–2014 研究助技師,中研院生化所 1992–2008 研究助理,中研院生化所 學歷 2001 博士,生物化學,國立臺灣大學 主要著作 IntermolecularbindingbetweenTIFA-FHAandTIFA-pTmediatestumornecrosisfactoralphastimulationandNF-kappaBactivation. HuangCC,WengJH,WeiTY,WuPY,HsuPH,ChenYH,WangSC,QinD,HungCC,ChenST,WangAH,ShyyJY,TsaiMD Molecularandcellularbiology(2012) PhosphorylationofthezebrafishM6Abatserine263contributestofilopodiumformationinPC12cellsandneuriteoutgrowthinzebrafishembryos. HuangKY,ChenGD,ChengCH,LiaoKY,HungCC,ChangGD,HwangPP,LinSY,TsaiMC,KhooKH,LeeMT,HuangCJ PloSone(2011) Synthesisandstructure-activityrelationshipsofnovelfurazan-3,4-diamideanalogsaspotentanti-canceragents. LiWS,MoreSV,WangCH,JenYC,YaoCF,WangTF,HungCC,JaoSC Bioorganic&medicinalchemistryletters(2010) Up-regulationoffibronectinandtissuetransglutaminasepromotescellinvasioninvolvingincreasedassociationwithintegrinandMMPexpressioninA431cells. ChenSH,LinCY,LeeLT,ChangGD,LeePP,HungCC,KaoWT,TsaiPH,SchallyAV,HwangJJ,LeeMT Anticancerresearch(2010) Recapitulationofzebrafishsncgaexpressionpatternandlabelingthehabenularcomplexintransgeniczebrafishusinggreenfluorescentproteinreportergene. ChenYC,ChengCH,ChenGD,HungCC,YangCH,HwangSP,KawakamiK,WuBK,HuangCJ Developmentaldynamics(2009) InvestigationofMMP-2and-9inahighlyinvasiveA431tumorcellsub-lineselectedfromaBoydenchamberassay. KaoWT,LinCY,LeeLT,LeePP,HungCC,LinYS,ChenSH,KeFC,HwangJJ,LeeMT Anticancerresearch(2008) Thezebrafisherythropoietin:functionalidentificationandbiochemicalcharacterization. ChuCY,ChengCH,ChenGD,ChenYC,HungCC,HuangKY,HuangCJ FEBSletters(2007) Requirementofnuclearlocalizationandtranscriptionalactivityofp53foritstargetingtotheyolksyncytiallayer(YSL)nucleiinzebrafishembryoanditsuseforapoptosisassay. ChenGD,ChouCM,HwangSP,WangFF,ChenYC,HungCC,ChenJY,HuangCJ Biochemicalandbiophysicalresearchcommunications(2006) Crystalstructureofaplatelet-agglutinatingfactorisolatedfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus). HuangKF,KoTP,HungCC,ChuJ,WangAH,ChiouSH TheBiochemicaljournal(2004) 3-phosphoinositide-dependentproteinkinase-1/Aktsignalingrepresentsamajorcyclooxygenase-2-independenttargetforcelecoxibinprostatecancercells. KulpSK,YangYT,HungCC,ChenKF,LaiJP,TsengPH,FowbleJW,WardPJ,ChenCS Cancerresearch(2004) 著作列表 ChouHY,LeeYT,LinYJ,WenJK,PengWH,HsiehPL,LinSY,(HungCC),ChenGC  (2021-10)  Autophagy  17(10),2750-2765  "PTPN9-mediateddephosphorylationofVTI1BpromotesATG16L1precursorfusionandautophagosomeformation." WenJK,WangYT,ChanCC,HsiehCW,LiaoHM,(HungCC),ChenGC  (2017-11)  eLife  6,e29338  "Atg9antagonizesTORsignalingtoregulateintestinalcellgrowthandepithelialhomeostasisinDrosophila" TsaiPH,ChengCH,LinCY,HuangYT,LeeLT,KandaswamiCC,LinYC,LeeKP,(HungCC),HwangJJ,KeFC,ChangGD,LeeMT  (2016-12)  Anticancerresearch  36(12),6367-6380  "DietaryFlavonoidsLuteolinandQuercetinSuppressedCancerStemCellPropertiesandMetastaticPotentialofIsolatedProstateCancerCells." LienHW,YuanRY,ChouCM,ChenYC,(HungCC),HuCH,HwangSP,HwangPP,ShenCN,ChenCL,ChengCH,HuangCJ  (2016-06)  Scientificreports  6,28297  "ZebrafishcyclinDxisrequiredfordevelopmentofmotorneuronprogenitors,anditsexpressionisregulatedbyhypoxia-induciblefactor2alpha." WuBK,YuanRY,LienHW,(HungCC),HwangPP,ChenRP,ChangCC,LiaoYF,HuangCJ  (2016-02)  Journalofbiomedicalscience  23,25  "Multiplesignalingfactorsanddrugsalleviateneuronaldeathinducedbyexpressionofhumanandzebrafishtauproteinsinvivo." YuanWC,LeeYR,LinSY,ChangLY,TanYP,(HungCC),KuoJC,LiuCH,LinMY,XuM,ChenZJ,ChenRH  (2014-05)  Molecularcell  54(4),586-600  "K33-LinkedPolyubiquitinationofCoronin7byCul3-KLHL20UbiquitinE3LigaseRegulatesProteinTrafficking." HanHW,ChouCM,ChuCY,ChengCH,YangCH,(HungCC),HwangPP,LeeSJ,LiaoYF,HuangCJ  (2014)  PloSone  9(1),e86345  "TheNogo-C2/Nogoreceptorcomplexregulatesthemorphogenesisofzebrafishlaterallineprimordiumthroughmodulatingtheexpressionofdkk1b,aWntsignalinhibitor." LienHW,YangCH,ChengCH,(HungCC),LiaoWH,HwangPP,HanYS,HuangCJ  (2013-09)  Internationaljournalofbiologicalsciences  9(9),872-886  "Anovelzincfingerprotein219-like(ZNF219L)isinvolvedintheregulationofcollagentype2alpha1a(col2a1a)geneexpressioninzebrafishnotochord." HuangCC,WengJH,WeiTY,WuPY,HsuPH,ChenYH,WangSC,QinD,(HungCC),ChenST,WangAH,ShyyJY,TsaiMD  (2012-07)  Molecularandcellularbiology  32(14),2664-2673  "IntermolecularbindingbetweenTIFA-FHAandTIFA-pTmediatestumornecrosisfactoralphastimulationandNF-kappaBactivation." HuangKY,ChenGD,ChengCH,LiaoKY,(HungCC),ChangGD,HwangPP,LinSY,TsaiMC,KhooKH,LeeMT,HuangCJ  (2011)  PloSone  6(10),e26461  "PhosphorylationofthezebrafishM6Abatserine263contributestofilopodiumformationinPC12cellsandneuriteoutgrowthinzebrafishembryos." ChenSH,LinCY,LeeLT,ChangGD,LeePP,(HungCC),KaoWT,TsaiPH,SchallyAV,HwangJJ,LeeMT  (2010-10)  Anticancerresearch  30(10),4177-4186  "Up-regulationoffibronectinandtissuetransglutaminasepromotescellinvasioninvolvingincreasedassociationwithintegrinandMMPexpressioninA431cells." LiWS,MoreSV,WangCH,JenYC,YaoCF,WangTF,(HungCC),JaoSC  (2010-02)  Bioorganic&medicinalchemistryletters  20(3),1148-1152  "Synthesisandstructure-activityrelationshipsofnovelfurazan-3,4-diamideanalogsaspotentanti-canceragents." ChenYC,ChengCH,ChenGD,HungCC,YangCH,HwangSP,KawakamiK,WuBK,HuangCJ  (2009)  Developmentaldynamics  238(3),746-754  "Recapitulationofzebrafishsncgaexpressionpatternandlabelingthehabenularcomplexintransgeniczebrafishusinggreenfluorescentproteinreportergene." KaoWT,LinCY,LeeLT,LeePP,HungCC,LinYS,ChenSH,KeFC,HwangJJ,LeeMT  (2008)  Anticancerresearch  28(4B),2109-2120  "InvestigationofMMP-2and-9inahighlyinvasiveA431tumorcellsub-lineselectedfromaBoydenchamberassay." ChuCY,ChengCH,ChenGD,ChenYC,HungCC,HuangKY,HuangCJ  (2007)  FEBSletters  581(22),4265-4271  "Thezebrafisherythropoietin:functionalidentificationandbiochemicalcharacterization." ChenGD,ChouCM,HwangSP,WangFF,ChenYC,HungCC,ChenJY,HuangCJ  (2006)  Biochemicalandbiophysicalresearchcommunications  344(1),272-282  "Requirementofnuclearlocalizationandtranscriptionalactivityofp53foritstargetingtotheyolksyncytiallayer(YSL)nucleiinzebrafishembryoanditsuseforapoptosisassay." KulpSK,YangYT,HungCC,ChenKF,LaiJP,TsengPH,FowbleJW,WardPJ,ChenCS  (2004)  Cancerresearch  64(4),1444-1451  "3-phosphoinositide-dependentproteinkinase-1/Aktsignalingrepresentsamajorcyclooxygenase-2-independenttargetforcelecoxibinprostatecancercells." HuangKF,KoTP,HungCC,ChuJ,WangAH,ChiouSH  (2004)  TheBiochemicaljournal  378(Pt2),399-407  "Crystalstructureofaplatelet-agglutinatingfactorisolatedfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus)." HungCC,ChiouSH  (2001)  Biochemicalandbiophysicalresearchcommunications  281(4),1012-1018  "FibrinogenolyticproteasesisolatedfromthesnakevenomofTaiwanhabu:serineproteaseswithkallikrein-likeandangiotensin-degradingactivities." HungCC,ChiouSH  (2000)  Biochemicalandbiophysicalresearchcommunications  275(3),924-930  "Expressionofakallikrein-likeproteasefromthesnakevenom:engineeringofautocatalyticsiteinthefusionproteintofacilitateproteinrefolding." JuanHF,HungCC,WangKT,ChiouSH  (1999)  Biochemicalandbiophysicalresearchcommunications  257(2),500-510  "Comparisonofthreeclassesofsnakeneurotoxinsbyhomologymodelingandcomputersimulationgraphics." HuangKF,HungCC,WuSH,ChiouSH  (1998)  Biochemicalandbiophysicalresearchcommunications  248(3),562-568  "CharacterizationofthreeendogenouspeptideinhibitorsformultiplemetalloproteinaseswithfibrinogenolyticactivityfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus)." LiaoJH,HungCC,LeeJS,WuSH,ChiouSH  (1998)  Biochemicalandbiophysicalresearchcommunications  244(1),131-137  "Characterization,cloning,andexpressionofporcinealphaBcrystallin." HungCC,WuSH,ChiouSH  (1998)  Journalofproteinchemistry  17(2),107-114  "Twonovelalpha-neurotoxinsisolatedfromTaiwancobra:sequencecharacterizationandphylogeneticcomparisonofhomologousneurotoxins." HuangKF,HungCC,PanFM,ChowLP,TsugitaA,ChiouSH  (1995)  Biochemicalandbiophysicalresearchcommunications  216(1),223-233  "CharacterizationofmultiplemetalloproteinaseswithfibrinogenolyticactivityfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus):proteinmicrosequencingcoupledwithcDNAsequenceanalysis." ChiouSH,HungCC,HuangHC,ChenST,WangKT,YangCC  (1995)  Biochemicalandbiophysicalresearchcommunications  206(1),22-32  "SequencecomparisonandcomputermodellingofcardiotoxinsandcobrotoxinisolatedfromTaiwancobra." HungCC,HuangKF,ChiouSH  (1994)  Biochemicalandbiophysicalresearchcommunications  205(3),1707-1715  "Characterizationofonenovelvenomproteasewithbeta-fibrinogenaseactivityfromtheTaiwanhabu(Trimeresurusmucrosquamatus):purificationandcDNAsequenceanalysis." HungCC,ChiouSH  (1994)  Biochemicalandbiophysicalresearchcommunications  201(3),1414-1423  "Isolationofmultipleisoformsofalpha-fibrinogenasefromtheWesterndiamondbackrattlesnake,Crotalusatrox:N-terminalsequencehomologywithancrod,anantithromboticagentfromMalayanviper." ChiouSH,HungCC,LinCW  (1992)  Biochimicaetbiophysicaacta  1160(3),317-324  "Biochemicalcharacterizationofcrystallinsfrompigeonlenses:structuralandsequenceanalysisofpigeondelta-crystallin." 研究領域與專長 學經歷 主要著作 著作列表 ProvidingtrainingandtechnicalexpertisetoIBCinvestigatorsinterestedinusingmicroscopy,flowcytometerandscientificimagingintheirresearch Modalitiesandtechniquesincludinglaserscanningconfocalmicroscopy,wide-fieldfluorescencemicroscopy,livecellimaging Indepthcollaborationonselectprojectsinvolvingadvancedmicroscopytechniquesandmethodologies Maintenanceoftheinstrumentationandassociatedinfrastructure AssistancewitheducationalmicroscopycoursesconductedatIBC 經歷 2014– 迄今 研究副技師,中研院生化所 2009–2014 研究助技師,中研院生化所 1992–2008 研究助理,中研院生化所 學歷 2001 博士,生物化學,國立臺灣大學 IntermolecularbindingbetweenTIFA-FHAandTIFA-pTmediatestumornecrosisfactoralphastimulationandNF-kappaBactivation. HuangCC,WengJH,WeiTY,WuPY,HsuPH,ChenYH,WangSC,QinD,HungCC,ChenST,WangAH,ShyyJY,TsaiMD Molecularandcellularbiology(2012) PhosphorylationofthezebrafishM6Abatserine263contributestofilopodiumformationinPC12cellsandneuriteoutgrowthinzebrafishembryos. HuangKY,ChenGD,ChengCH,LiaoKY,HungCC,ChangGD,HwangPP,LinSY,TsaiMC,KhooKH,LeeMT,HuangCJ PloSone(2011) Synthesisandstructure-activityrelationshipsofnovelfurazan-3,4-diamideanalogsaspotentanti-canceragents. LiWS,MoreSV,WangCH,JenYC,YaoCF,WangTF,HungCC,JaoSC Bioorganic&medicinalchemistryletters(2010) Up-regulationoffibronectinandtissuetransglutaminasepromotescellinvasioninvolvingincreasedassociationwithintegrinandMMPexpressioninA431cells. ChenSH,LinCY,LeeLT,ChangGD,LeePP,HungCC,KaoWT,TsaiPH,SchallyAV,HwangJJ,LeeMT Anticancerresearch(2010) Recapitulationofzebrafishsncgaexpressionpatternandlabelingthehabenularcomplexintransgeniczebrafishusinggreenfluorescentproteinreportergene. ChenYC,ChengCH,ChenGD,HungCC,YangCH,HwangSP,KawakamiK,WuBK,HuangCJ Developmentaldynamics(2009) InvestigationofMMP-2and-9inahighlyinvasiveA431tumorcellsub-lineselectedfromaBoydenchamberassay. KaoWT,LinCY,LeeLT,LeePP,HungCC,LinYS,ChenSH,KeFC,HwangJJ,LeeMT Anticancerresearch(2008) Thezebrafisherythropoietin:functionalidentificationandbiochemicalcharacterization. ChuCY,ChengCH,ChenGD,ChenYC,HungCC,HuangKY,HuangCJ FEBSletters(2007) Requirementofnuclearlocalizationandtranscriptionalactivityofp53foritstargetingtotheyolksyncytiallayer(YSL)nucleiinzebrafishembryoanditsuseforapoptosisassay. ChenGD,ChouCM,HwangSP,WangFF,ChenYC,HungCC,ChenJY,HuangCJ Biochemicalandbiophysicalresearchcommunications(2006) Crystalstructureofaplatelet-agglutinatingfactorisolatedfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus). HuangKF,KoTP,HungCC,ChuJ,WangAH,ChiouSH TheBiochemicaljournal(2004) 3-phosphoinositide-dependentproteinkinase-1/Aktsignalingrepresentsamajorcyclooxygenase-2-independenttargetforcelecoxibinprostatecancercells. KulpSK,YangYT,HungCC,ChenKF,LaiJP,TsengPH,FowbleJW,WardPJ,ChenCS Cancerresearch(2004) ChouHY,LeeYT,LinYJ,WenJK,PengWH,HsiehPL,LinSY,(HungCC),ChenGC  (2021-10)  Autophagy  17(10),2750-2765  "PTPN9-mediateddephosphorylationofVTI1BpromotesATG16L1precursorfusionandautophagosomeformation." WenJK,WangYT,ChanCC,HsiehCW,LiaoHM,(HungCC),ChenGC  (2017-11)  eLife  6,e29338  "Atg9antagonizesTORsignalingtoregulateintestinalcellgrowthandepithelialhomeostasisinDrosophila" TsaiPH,ChengCH,LinCY,HuangYT,LeeLT,KandaswamiCC,LinYC,LeeKP,(HungCC),HwangJJ,KeFC,ChangGD,LeeMT  (2016-12)  Anticancerresearch  36(12),6367-6380  "DietaryFlavonoidsLuteolinandQuercetinSuppressedCancerStemCellPropertiesandMetastaticPotentialofIsolatedProstateCancerCells." LienHW,YuanRY,ChouCM,ChenYC,(HungCC),HuCH,HwangSP,HwangPP,ShenCN,ChenCL,ChengCH,HuangCJ  (2016-06)  Scientificreports  6,28297  "ZebrafishcyclinDxisrequiredfordevelopmentofmotorneuronprogenitors,anditsexpressionisregulatedbyhypoxia-induciblefactor2alpha." WuBK,YuanRY,LienHW,(HungCC),HwangPP,ChenRP,ChangCC,LiaoYF,HuangCJ  (2016-02)  Journalofbiomedicalscience  23,25  "Multiplesignalingfactorsanddrugsalleviateneuronaldeathinducedbyexpressionofhumanandzebrafishtauproteinsinvivo." YuanWC,LeeYR,LinSY,ChangLY,TanYP,(HungCC),KuoJC,LiuCH,LinMY,XuM,ChenZJ,ChenRH  (2014-05)  Molecularcell  54(4),586-600  "K33-LinkedPolyubiquitinationofCoronin7byCul3-KLHL20UbiquitinE3LigaseRegulatesProteinTrafficking." HanHW,ChouCM,ChuCY,ChengCH,YangCH,(HungCC),HwangPP,LeeSJ,LiaoYF,HuangCJ  (2014)  PloSone  9(1),e86345  "TheNogo-C2/Nogoreceptorcomplexregulatesthemorphogenesisofzebrafishlaterallineprimordiumthroughmodulatingtheexpressionofdkk1b,aWntsignalinhibitor." LienHW,YangCH,ChengCH,(HungCC),LiaoWH,HwangPP,HanYS,HuangCJ  (2013-09)  Internationaljournalofbiologicalsciences  9(9),872-886  "Anovelzincfingerprotein219-like(ZNF219L)isinvolvedintheregulationofcollagentype2alpha1a(col2a1a)geneexpressioninzebrafishnotochord." HuangCC,WengJH,WeiTY,WuPY,HsuPH,ChenYH,WangSC,QinD,(HungCC),ChenST,WangAH,ShyyJY,TsaiMD  (2012-07)  Molecularandcellularbiology  32(14),2664-2673  "IntermolecularbindingbetweenTIFA-FHAandTIFA-pTmediatestumornecrosisfactoralphastimulationandNF-kappaBactivation." HuangKY,ChenGD,ChengCH,LiaoKY,(HungCC),ChangGD,HwangPP,LinSY,TsaiMC,KhooKH,LeeMT,HuangCJ  (2011)  PloSone  6(10),e26461  "PhosphorylationofthezebrafishM6Abatserine263contributestofilopodiumformationinPC12cellsandneuriteoutgrowthinzebrafishembryos." ChenSH,LinCY,LeeLT,ChangGD,LeePP,(HungCC),KaoWT,TsaiPH,SchallyAV,HwangJJ,LeeMT  (2010-10)  Anticancerresearch  30(10),4177-4186  "Up-regulationoffibronectinandtissuetransglutaminasepromotescellinvasioninvolvingincreasedassociationwithintegrinandMMPexpressioninA431cells." LiWS,MoreSV,WangCH,JenYC,YaoCF,WangTF,(HungCC),JaoSC  (2010-02)  Bioorganic&medicinalchemistryletters  20(3),1148-1152  "Synthesisandstructure-activityrelationshipsofnovelfurazan-3,4-diamideanalogsaspotentanti-canceragents." ChenYC,ChengCH,ChenGD,HungCC,YangCH,HwangSP,KawakamiK,WuBK,HuangCJ  (2009)  Developmentaldynamics  238(3),746-754  "Recapitulationofzebrafishsncgaexpressionpatternandlabelingthehabenularcomplexintransgeniczebrafishusinggreenfluorescentproteinreportergene." KaoWT,LinCY,LeeLT,LeePP,HungCC,LinYS,ChenSH,KeFC,HwangJJ,LeeMT  (2008)  Anticancerresearch  28(4B),2109-2120  "InvestigationofMMP-2and-9inahighlyinvasiveA431tumorcellsub-lineselectedfromaBoydenchamberassay." ChuCY,ChengCH,ChenGD,ChenYC,HungCC,HuangKY,HuangCJ  (2007)  FEBSletters  581(22),4265-4271  "Thezebrafisherythropoietin:functionalidentificationandbiochemicalcharacterization." ChenGD,ChouCM,HwangSP,WangFF,ChenYC,HungCC,ChenJY,HuangCJ  (2006)  Biochemicalandbiophysicalresearchcommunications  344(1),272-282  "Requirementofnuclearlocalizationandtranscriptionalactivityofp53foritstargetingtotheyolksyncytiallayer(YSL)nucleiinzebrafishembryoanditsuseforapoptosisassay." KulpSK,YangYT,HungCC,ChenKF,LaiJP,TsengPH,FowbleJW,WardPJ,ChenCS  (2004)  Cancerresearch  64(4),1444-1451  "3-phosphoinositide-dependentproteinkinase-1/Aktsignalingrepresentsamajorcyclooxygenase-2-independenttargetforcelecoxibinprostatecancercells." HuangKF,KoTP,HungCC,ChuJ,WangAH,ChiouSH  (2004)  TheBiochemicaljournal  378(Pt2),399-407  "Crystalstructureofaplatelet-agglutinatingfactorisolatedfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus)." HungCC,ChiouSH  (2001)  Biochemicalandbiophysicalresearchcommunications  281(4),1012-1018  "FibrinogenolyticproteasesisolatedfromthesnakevenomofTaiwanhabu:serineproteaseswithkallikrein-likeandangiotensin-degradingactivities." HungCC,ChiouSH  (2000)  Biochemicalandbiophysicalresearchcommunications  275(3),924-930  "Expressionofakallikrein-likeproteasefromthesnakevenom:engineeringofautocatalyticsiteinthefusionproteintofacilitateproteinrefolding." JuanHF,HungCC,WangKT,ChiouSH  (1999)  Biochemicalandbiophysicalresearchcommunications  257(2),500-510  "Comparisonofthreeclassesofsnakeneurotoxinsbyhomologymodelingandcomputersimulationgraphics." HuangKF,HungCC,WuSH,ChiouSH  (1998)  Biochemicalandbiophysicalresearchcommunications  248(3),562-568  "CharacterizationofthreeendogenouspeptideinhibitorsformultiplemetalloproteinaseswithfibrinogenolyticactivityfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus)." LiaoJH,HungCC,LeeJS,WuSH,ChiouSH  (1998)  Biochemicalandbiophysicalresearchcommunications  244(1),131-137  "Characterization,cloning,andexpressionofporcinealphaBcrystallin." HungCC,WuSH,ChiouSH  (1998)  Journalofproteinchemistry  17(2),107-114  "Twonovelalpha-neurotoxinsisolatedfromTaiwancobra:sequencecharacterizationandphylogeneticcomparisonofhomologousneurotoxins." HuangKF,HungCC,PanFM,ChowLP,TsugitaA,ChiouSH  (1995)  Biochemicalandbiophysicalresearchcommunications  216(1),223-233  "CharacterizationofmultiplemetalloproteinaseswithfibrinogenolyticactivityfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus):proteinmicrosequencingcoupledwithcDNAsequenceanalysis." ChiouSH,HungCC,HuangHC,ChenST,WangKT,YangCC  (1995)  Biochemicalandbiophysicalresearchcommunications  206(1),22-32  "SequencecomparisonandcomputermodellingofcardiotoxinsandcobrotoxinisolatedfromTaiwancobra." HungCC,HuangKF,ChiouSH  (1994)  Biochemicalandbiophysicalresearchcommunications  205(3),1707-1715  "Characterizationofonenovelvenomproteasewithbeta-fibrinogenaseactivityfromtheTaiwanhabu(Trimeresurusmucrosquamatus):purificationandcDNAsequenceanalysis." HungCC,ChiouSH  (1994)  Biochemicalandbiophysicalresearchcommunications  201(3),1414-1423  "Isolationofmultipleisoformsofalpha-fibrinogenasefromtheWesterndiamondbackrattlesnake,Crotalusatrox:N-terminalsequencehomologywithancrod,anantithromboticagentfromMalayanviper." ChiouSH,HungCC,LinCW  (1992)  Biochimicaetbiophysicaacta  1160(3),317-324  "Biochemicalcharacterizationofcrystallinsfrompigeonlenses:structuralandsequenceanalysisofpigeondelta-crystallin." 洪金俊博士 研究副技師 中央研究院生物化學研究所406室 E-mail:[email protected] Tel:+886-2-2785-5696,4022 Curriculumvitae ResearchInterests ProvidingtrainingandtechnicalexpertisetoIBCinvestigatorsinterestedinusingmicroscopy,flowcytometerandscientificimagingintheirresearch Modalitiesandtechniquesincludinglaserscanningconfocalmicroscopy,wide-fieldfluorescencemicroscopy,livecellimaging Indepthcollaborationonselectprojectsinvolvingadvancedmicroscopytechniquesandmethodologies Maintenanceoftheinstrumentationandassociatedinfrastructure AssistancewitheducationalmicroscopycoursesconductedatIBC Experience/Education 經歷 2014– 迄今 研究副技師,中研院生化所 2009–2014 研究助技師,中研院生化所 1992–2008 研究助理,中研院生化所 學歷 2001 博士,生物化學,國立臺灣大學 SelectedPublications IntermolecularbindingbetweenTIFA-FHAandTIFA-pTmediatestumornecrosisfactoralphastimulationandNF-kappaBactivation. HuangCC,WengJH,WeiTY,WuPY,HsuPH,ChenYH,WangSC,QinD,HungCC,ChenST,WangAH,ShyyJY,TsaiMD Molecularandcellularbiology(2012) PhosphorylationofthezebrafishM6Abatserine263contributestofilopodiumformationinPC12cellsandneuriteoutgrowthinzebrafishembryos. HuangKY,ChenGD,ChengCH,LiaoKY,HungCC,ChangGD,HwangPP,LinSY,TsaiMC,KhooKH,LeeMT,HuangCJ PloSone(2011) Synthesisandstructure-activityrelationshipsofnovelfurazan-3,4-diamideanalogsaspotentanti-canceragents. LiWS,MoreSV,WangCH,JenYC,YaoCF,WangTF,HungCC,JaoSC Bioorganic&medicinalchemistryletters(2010) Up-regulationoffibronectinandtissuetransglutaminasepromotescellinvasioninvolvingincreasedassociationwithintegrinandMMPexpressioninA431cells. ChenSH,LinCY,LeeLT,ChangGD,LeePP,HungCC,KaoWT,TsaiPH,SchallyAV,HwangJJ,LeeMT Anticancerresearch(2010) Recapitulationofzebrafishsncgaexpressionpatternandlabelingthehabenularcomplexintransgeniczebrafishusinggreenfluorescentproteinreportergene. ChenYC,ChengCH,ChenGD,HungCC,YangCH,HwangSP,KawakamiK,WuBK,HuangCJ Developmentaldynamics(2009) InvestigationofMMP-2and-9inahighlyinvasiveA431tumorcellsub-lineselectedfromaBoydenchamberassay. KaoWT,LinCY,LeeLT,LeePP,HungCC,LinYS,ChenSH,KeFC,HwangJJ,LeeMT Anticancerresearch(2008) Thezebrafisherythropoietin:functionalidentificationandbiochemicalcharacterization. ChuCY,ChengCH,ChenGD,ChenYC,HungCC,HuangKY,HuangCJ FEBSletters(2007) Requirementofnuclearlocalizationandtranscriptionalactivityofp53foritstargetingtotheyolksyncytiallayer(YSL)nucleiinzebrafishembryoanditsuseforapoptosisassay. ChenGD,ChouCM,HwangSP,WangFF,ChenYC,HungCC,ChenJY,HuangCJ Biochemicalandbiophysicalresearchcommunications(2006) Crystalstructureofaplatelet-agglutinatingfactorisolatedfromthevenomofTaiwanhabu(Trimeresurusmucrosquamatus). HuangKF,KoTP,HungCC,ChuJ,WangAH,ChiouSH TheBiochemicaljournal(2004) 3-phosphoinositide-dependentproteinkinase-1/Aktsignalingrepresentsamajorcyclooxygenase-2-independenttargetforcelecoxibinprostatecancercells. KulpSK,YangYT,HungCC,ChenKF,LaiJP,TsengPH,FowbleJW,WardPJ,ChenCS Cancerresearch(2004) 中文 中文English 簡介 生化所歷史 組織架構 歷任所長 學術諮詢委員 位置圖 保有個人資料檔案公開項目彙整表 人員 研究人員 專任研究人員 王彥士 史有伶 安形高志 何孟樵 吳世雄 吳昆峯 呂桐睿 孟子青 林俊宏 林曉青 邱繼輝 姚季光 凌嘉鴻 徐尚德 張瀞仁 張崇毅 梁博煌 陳光超 陳宏文 陳佩燁 陳瑞華 楊維元 廖俊智 合聘研究人員 MatthiasWolf 余榮熾 翁啟惠 張茂山 游景晴 管永恕 冀宏源 蕭超隆 閻雲 通信研究人員 退休研究人員 研究技術人員 何孟儒 吳文晉 林淑妤 林聖偉 洪金俊 凃智傑 張淵智 黃開發 饒淑娟 訪問學者 王惠鈞 別所義隆 馬左仲 蔡明道 編制內助理 行政人員 研究 研究領域 精彩研究成果 生化所學人傑出成果 最新發表論文 設施 蛋白質設施 蛋白純化與離心機 哺乳類細胞重組蛋白表現 生物物理 蛋白質養晶 細胞生物設施 生物成像 組織病理 動物房 生物資訊 化學設施 合成 核磁共振 高效能液相層析 臺大生化所核心設施 全院性核心設施 蛋白體與蛋白修飾分析公用質譜儀設施 生物物理核心設施 冷凍電子顯微鏡設施 整合型蛋白質結構與功能分析核心設施 學術活動 生化所行事曆 演講研討會公告 近期消息 活動報導 學程 臺大生化科學研究所 國際研究生化學生物學與分子生物物理學學程 大專生暑期生化實習計畫 徵才 所內專區 相關連結 中央研究院 台灣蛋白質計畫(TPP) 中研院生命科學圖書館 學術活動中心訂房系統