張英峯副教授| 臺大植科所| 臺北市

E-mail: [email protected] 電話: (02) 33662534 專長: 植物蛋白質體學、植物生物化學學歷: 美國加州大學河濱分校博士研究室: 生命科學館R905.  張英峯副教授E-mail:[email protected] 電話:(02)33662534 專長:植物蛋白質體學、植物生物化學、分子生物學 學歷:美國加州大學河濱分校博士 研究室:生命科學館R905近年研究主題鹽分逆境下之植物反應及蛋白質磷酸化之變化 植物如何調控根生長發育研究室簡介 植物在鹽分逆境下，為求生存，會調控轉錄因子(transcriptionfactor)、轉譯因子、膜蛋白質受體(membranereceptor)等重要基因之表現。

鹽分逆境下尤其植物細胞的膜上一些膜蛋白質(如鈣離子通道)活性會受不同程度的磷酸化調控，蛋白質磷酸化在訊號傳遞上扮演重要之角色，通常經由專一性激酶催化。

本實驗室利用阿拉伯芥為模式生物，利用生化遺傳分析方法鑽研特定蛋白質磷酸化在鹽分逆境及生長發育之調控機制及功能。

此外，植物根部生長發育受內部代謝物或荷爾蒙及外部環境因子影響，本實驗室透過篩選根部生長發育缺陷之突變株，試圖找出造成表型不同的機制，並探究這些基因在鹽分逆境下如何影響植物生理。

圖中顯示在鹽分逆境下阿拉伯芥麩氨酸受體3.7之突變株glr3.7-2之細胞質鈣離子濃度較低代表著作A.期刊論文 LinWC,ChenYH,GuSY,ShenHL,HuangKC,LinWD,ChangMC,ChangIF,HongCY,ChengWH.*2021.CFM6isanessentialCRMproteinrequiredforthesplicingofnad5transcriptinArabidopsismitochondria.PlantCellPhysiol. ChenPY,HsuCY,LeeCE,ChangI.F.*2021.ArabidopsisglutamatereceptorGLR3.7isinvolvedinabscisicacidresponse.PlantSignalBehav. Wang,P.H.,Lee,C.E.,Lin,Y.S.,Lee,M.H.,Chen,P.Y.,Chang,H.C.,Chang,I.F.*2019.TheGlutamateReceptor-LikeProteinGLR3.7InteractsWith14-3-3ωandParticipatesinSaltStressResponseinArabidopsisthaliana.FrontiersinPlantScience10:1169 Chang,H.C.,Tsai,M.C.,Wu,S.S.,Chang,I.F.*2019.RegulationofABI5expressionbyABF3duringsaltstressresponsesinArabidopsisthaliana.BotanicalStudies60:16. Shih,Y.J.,Chang,H.C.,Tsai,M.C.,Wu,T.Y.,Wu,T.C.,PingKao,P.,Wen-YuanKao,W.Y.,Chang,I.F.*2018.Comparativeleafproteomicprofilingofsalt-treatednaturalvariantsofImperatacylindrica.Taiwania63:171-182. Singh,S.K.,Chien,C.T.,Chang,I,.F.*2016.TheArabidopsisglutamatereceptor-likegeneGLR3.6controlsrootdevelopmentbyrepressingtheKip-relatedproteingeneKRP4.JournalofExperimentalBotany67(6):1853-69.(SCI) Wu,T.Y.,Kao,P.,Chang,C.L.,Hsu,P.H.,Chou,C.H.,Chang,I,.F.*2015.PhosphoproteomicprofilingofmicrosomalfractionsinleavesofCogongrass(Imperatacylindrica).PlantOMICS(SCI) Lee,T.C.,Xiong,W.,Paddock,T.,Carrieri,D.,Chang,I.F.,Chiu,H.F.,Ungerer,J.,Juo,S.H.,Maness,P.C.,Yu,J.2015.Engineeredxyloseutilizationenhancesbio-productsproductivityinthecyanobacteriumSynechocystissp.PCC6803.MetabolicEngineering30:179-189.(SCI) Chen,Y.T.,Shen,C.H.,Lin,W.D.,Chu,H.A.,Huang,B.L.,Kuo,C.I.,Yeh,K.W.,Huang,L.C.,Chang,I.F.2013.SmallRNAsofSequoiasempervirensduringrejuvenationandphasechange.PlantBiology15:27-36.(SCI) Huang,S.J.,Chang,C.L.,Wang,P.H.,Tsai,M.C.,Hsu,P.H.,Chang,I.F.*2013.AtypeIIIACCsynthase,ACS7,isinvolvedinrootgravitropisminArabidopsisthaliana.JournalofExperimentalBotany64:4343-4360.(SCI) Chen,Y.T.,Shen,C.H.,Lin,W.D.,Chu,H.A.,Huang,B.L.,Kuo,C.I.,Yeh,K.W.,Huang,L.C.,Chang,I.F.2013.SmallRNAsofSequoiasempervirensduringrejuvenationandphasechange.PlantBiology15:27-36.(SCI) Chu,H.A.,Chang,I.F.,Shen,C.H.,Chen,Y.T.,Wang,H.T.,Huang,L.C.,Yeh,K.W.2012.Photosyntheticpropertiesandphotosystemstoichiometryofinvitro-grownjuvenile,adult,andrejuvenatedSequoiasempervirens(D.Don)Endl.BotanicalStudies53:223-227.(SCI) Chang,I.F.*,Hsu,J.L.,Hsu,P.H.,Sheng,W.A.,Lai,S.J.,Lee,C.,Chen,C.W.,Hsu,J.C.,Wang,S.Y.,Wang,L.Y.,Chen,C.C.2012.ComparativephosphoproteomicanalysisofmicrosomalfractionsofArabidopsisthalianaandOryzasativasubjectedtohighsalinity.PlantScience185-186:131-142.(SCI) Curran,A.+,Chang,I.F.+,Chang,C.L.Garg,S.,Garg,S.,Miguel,R.M.,Barron,Y.D.,Li,Y.,Romanowsky,S.,Cushman,J.C.,Gribskov,M.,Harmon,A.C.,Harper,J.F.*2011.Calcium-dependentproteinkinasesfromArabidopsisshowsubstratespecificitydifferencesinananalysisof103substrates.FruntPlantScience2:36(+:equalcontributions)(SCI) Chang,I.F.,Chen,P.J.,Shen,C.H.,Hsieh,T.J.,Hsu,Y.W.,Huang,B.L.,Kuo,C.I.,Chen,Y.T.,Chu,H.A.,Yeh,K.W.,Huang,L.C.*2010.ProteomicprofilingofproteinsassociatedwiththerejuvenationofSequoiasempervirens(D.Don)Endl.ProteomeScience8:64.(SCI) Hsu,J.L.,Wang,L.Y.,Wang,S.Y.,Lin,C.H.,Ho,K.C.,Shi,F.K.andChang,I.F.*2009.Functionalphosphoproteomicprofilingofphosphorylationsitesinmembranefractionsofsalt-stressedArabidopsisthaliana.ProteomeScience7:42.(SCI) Chang，I.F.+*,CurranA.+,WoolseyR.,QuiliciD.,CushmanJ.,MittlerR.,HarmonA.,HarperJ.F.*2009.Proteomicprofilingoftandemaffinitypurified14-3-3proteincomplexesinArabidopsisthaliana.Proteomics9:2697-2985.(+:equalcontributions)(SCI) Hsu,Y.W.,Sihgh,S.K.,Chiang,M.Y.,Wu,Y.Y.,Chang,I.F.2009.Strategiestolowergreenhousegaslevelbyriceagriculture.AfricanJournalofBiotechnology8:126-132. Chang,I.F.2008.EcotypicvariationofamedicinalplantImperatacylindricapopulationsinTaiwan:massspectrometry-basedproteomicevidence.JournalofMedicinalPlantsResearch2:71-76. Hsiao,H.Y.,Chang,I.F.2008.Proteomicprofilingofratbraindischargedbyultrasoundassociatedwithhighfrequencyelectro-magneticfield.ProteomicsResearchJournal1:41-53. Chang,I.-F.2006.Mass-spectrometrybasedproteomicanalysisoftheepitope-tagaffinitypurifiedproteincomplexineukaryotes. Proteomics 6:6158-6166(Review).(SCI) Chang,I.-F.andChou,C.H.2006.EcotypicvariationofImperatacylindricapopulationsinTaiwan:II.Physiologicalandbiochemicalevidences. BotanicalStudies 47:175-184.(SCI) RodriguezMilla,M.A.,Uno,Y.,Chang,I.–F.,Townsend,J.,Maher,E.A.,Quilici,D.andCushmanJ.C.2006.ArabidopsisAtCPK11,acalcium-dependentproteinkinase,phosphorylatesAtDi19,anuclearzincfingerprotein. FEBSLetters 580:904-911.(SCI) RodriguezMilla,M.A.,Townsend,J., Chang,I.–F. andCushman,J.C.2006.ArabidopsisDi19-RelatedGenesEncodeaNovelFamilyofProteinsWithTwoUnusualCys2/His2Zinc-FingerMotifsEvolutionaryConservedInvolvedinABA-IndependentStress-SignalingPathways. PlantMolecularBiology 61:13-30.(SCI) Chang,I.–F.,Hsiao,H.Y.2005.InductionofRhoGAPandpathologicalchangescharacteristicofAlzheimer’sdiseasebyUAHFEMFdischargeinratbrain.CurrentAlzheimerResearch 2:559-569.(SCI) Zanetti,M.E.,Chang,I.-F.,Galbraith,D.W.,Bailey-Serres,J.2005.ImmunopurificationofpolyribosomalcomplexesofArabidopsisforglobalanalysisofgeneexpression. PlantPhysiology 138:624-635.(SCI) Chang,I.-F.*,Szick-Miranda*,K.,Pan,S.,Bailey-Serres,J.2005.(*:equalcontributions)ProteomicCharacterizationofEvolutionarilyConservedandVariableProteinsofArabidopsisCytosolicRibosomes. PlantPhysiology 137:848-862.(SCI) Yang,C.M.,Wang,M.C.,Lu,Y.F.,Chang,I.-F.,Chou,C.H.2004.Humicsubstancesaffecttheactivityofchlorophyllase. JournalofChemicalEcology 30:1057-1065.(SCI) Yang,C.M.,Chou,C.H., Chang,I.-F.andLin,S.J.2004.Effectsofthreeallelopathicphenolicsonchlorophyllaccumulationofrice(Oryzasativa)seedlings:IIStimulationofconsumption-orientation. Bot.Bull.Acad.Sin. 45:119-125.(SCI) Williams,A.*,Werner-Fraczek.J.*, Chang,I.-F.,*Bailey-Serres,J.(*:equalcontributions)2003.Regulatedphosphorylationof40SribosomalS6inroottipsofmaize.PlantPhysiology 132:2086-2097.(SCI) Barakat,A.,Szick-Miranda,K., Chang,I.-F.,Guyot,R.,Blanc,G.,Cooke,R.,Delseny,M.,Bailey-Serres,J.2001.TheorganizationofcytoplasmicribosomalproteingenesintheArabidopsisgenome. PlantPhysiology 127:398-415.(SCI). B.著作 Kao,P.,Wu,T.Y.,Chang,C.L.,Chou,C.H.,Chang,I.F.2011.DecreasingofPopulationSizeofImperatacylindricaMangroveEcotype&Sea-LevelRising,GlobalWarmingImpacts-CaseStudiesontheEconomy,HumanHealth,andonUrbanandNaturalEnvironments,StefanoCasalegno(Ed.),ISBN:978-953-307-785-7,InTech.開設課程 普通生物學 蛋白質體學 植物生理學 生物技術核心實驗 進階植物分子生物學