林水龍教授

職 稱：教授; 學 歷：台大臨床醫學研究所博士、美國哈佛大學腎臟免疫博士後研究、台北醫學大學醫學士; 專 長：腎臟生理學; 聯絡電話：(02)23123456 ext. 成員簡介 生理所主任 專任與合聘教師 名譽與兼任教師 助教與職員 博士班 碩士班 林水龍教授 林水龍 職  稱：教授 學  歷：台大臨床醫學研究所博士、美國哈佛大學腎臟免疫博士後研究、台北醫學大學醫學士 專  長：腎臟生理學 聯絡電話：(02)23123456ext.88235 E-mail：[email protected] 研究方向 腎臟與高血壓 研究方向                        我們的主要興趣在研究腎臟相關疾病，老化與併發症的病理機轉及其治療。

台灣的慢性腎病病患需要透析治療的盛行率與發生率都高居世界第一，各種急性腎損傷或急性腎病的巨大醫療支出與併發症也是全球的重要課題。

我們有這個必要，也有這種優勢可以在這領域深入研究。

   在整合病患的臨床資料後，我們利用動物實驗以證實其因果關係並尋找新穎治療。

本實驗室主要的研究工具為基因改造或基因轉殖鼠，利用reportermice或特定腎臟細胞之特定基因於特定時間進行基因剔除以觀察其對於腎臟再生/修復/纖維化/老化/腫瘤，腎臟病合併症，透析造成腹膜炎/腹膜硬化的影響，以及研發次世代的腎臟替代療法。

研究的細胞包括腎臟血管周邊細胞(pericytes)，腎臟小管上皮細胞(renaltubularepithelialcells)，腎小球足細胞(podocytes)，腹膜上皮細胞(mesothelialcells)，腹膜下纖維母細胞(submesothelialfibroblasts)，巨嗜細胞(macrophages)，淋巴球細胞(lymphocytes)，血管內皮細胞(endothelialcells)等。

有持續關注的分子包括促紅血球生成素(erythropoietin)，低氧誘發因子(hypoxia-induciblefactors),甲基移轉酶(methyltransferase),血管生成素(angiopoietin)，第二型血管張力素(angiotensinII)，荃固酮(aldosterone)，與galectin-3(半乳糖凝集素-3)等。

研究主題 表基因調控腎臟血管周邊細胞。

腎臟細胞的交互作用在腎臟損傷與修護的角色。

腹膜細胞的交互作用在腹膜損傷與修護的角色。

慢性腎病相關的心血管疾病病理機轉與治療。

腎臟老化的機轉。

T淋巴球分化與功能。

次世代的腎臟替代療法。

實驗室技術 基因改造鼠:Cre-loxPsystem,transgenicmice 動物模式:organfibrosis,peritonitis,acutekidneyinjury,bonemarrowtransplantation,parabiosis 病毒載體技術:adenovirus,retrovirus,lentivirus 細胞培養:kidneycells,bonemarrowderivedmacrophages,endothelialcells,mesothelialcells,fibroblasts 分子生物學:RNA,DNA,protein,epigenetic 組織免疫學:immunohistochemistry,immunofluorescence 學術著作 Publicationlist ChenYT,HsuH,LinCC,PanSY,LiuSY,WuCF,TsaiPZ,LiaoCT,ChengHT,ChiangWC,ChenYM,ChuTS,LinSL(2020,Jan).InflammatorymacrophagesswitchtoCCL17-expressingphenotypeandpromoteperitonealfibrosis.JPathol,250(1):55-66. ChiangWC,HuangYC,FuTI,ChenPM,ChangFC,LaiCF,WuVC,LinSL,ChenYM(2019,Feb).Angiopoietin1influencesischemicreperfusionrenalinjuryviamodulatingendotheliumsurvivalandregeneration.MolMed,25(1):5. ShihHM,WuCJ,LinSL(2018,Nov).Physiologyandpathophysiologyofrenalerythropoietin-producingcells.JFormosMedAssoc,117(11):955-963. LinYF,LinSL,HuangTM,YangSY,LaiTS,ChenL,WuVC,ChuTS,WuKD;NationalTaiwanUniversityHospitalStudyGrouponAcuteRenalFailure(NSARF)(2018,Oct).New-onsetdiabetesafteracutekidneyinjuryrequiringdialysis.DiabetesCare,41(10):2105-2110. ShihYC,ChenCL,ZhangY,MellorRL,KanterEM,FangY,WangHC,HungCT,NongJY,ChenHJ,LeeTH,TsengYS,ChenCN,WuCC,LinSL,YamadaKA,NerbonneJM,YangKC(2018,Apr).EndoplasmicreticulumproteinTXNDC5augmentsmyocardialfibrosisbyfacilitatingextracellularmatrixproteinfoldingandredox-sensitivecardiacfibroblastactivation.CircRes,122(8):1062-1068. ChouYH,HuangTM,PanSY,ChangCH,LaiCF,WuVC,WuMS,WuKD,ChuTS,LinSL(2017,Apr).Renin-angiotensinsysteminhibitorisassociatedwithlowerriskofensuingchronickidneydiseaseafterfunctionalrecoveryfromacutekidneyinjury.SciRep7:46518. PanSY,ChiangWC,ChenPM,LiuHH,ChouYH,LaiTS,LaiCF,ChiuYL,LinWY,ChenYM,ChuTS,LinSL(2017,Mar).restricteduseoferythropoiesis-stimulatingagentissafeandassociatedwithdeferreddialysisinitiationinstage5chronickidneydisease.SciRep7:44013 ChengSY,ChouYH,LiaoFL,LinCC,ChangFC,LiuCH,HuangTM,LaiCF,LinYF,WuVC,ChuTS,WuMS,LinSL.(2016,Sep).Losartanreducesensuingchronickidneydiseaseandmortalityafteracutekidneyinjury.SciRep6:34265. ChangYT,YangCC,PanSY,ChouYH,ChangFC,LaiCF,TsaiMH,HsuHL,LinCH,ChiangWC,WuMS,ChuTS,ChenYM,LinSL(2016,Feb).DNAmethyltransferaseinhibitionrestoreserythropoietinproductioninfibroticmurinekidneys.JClinInvest,126(2):721-731. ChenYT,ChangYT,PanSY,ChouYH,ChangFC,YehPY,LiuYH,ChiangWC,ChenYM,WuKD,TsaiTJ,DuffieldJS,LinSL(2014,Dec).Lineagetracingrevealsdistinctivefatesformesothelialcellsandsubmesothelialfibroblastsduringperitonealinjuryinmice.JAmSocNephrol,25:2847–2858. ChangFC,ChiangWC,TsaiMH,ChouYH,PanSY,ChangYT,YehPY,ChenYT,ChiangCK,ChenYM,ChuTS,WuKD,LinSL(2014,Jun).Angiopoietin2InducedArterialStiffnessinChronicKidneyDisease.JAmSocNephrol,25:1198-1209. RenS,JohnsonB,KidaY,IpC,DavidsonKC,LinSL,KobayashiA,LangRA,HadjantonakisAK,MoonRT,DuffieldJS(2013,Jan).LRP-6isco-receptorformultiplefibrogenicsignalingpathwaysinpericytesandmyofibroblaststhatareinhibitedbyDKK-1.ProcNatlAcadSciUSA,110:1440-1445. WuCF,ChiangWC,LaiCF,ChangFC,ChenYT,ChouYH,WuTH,LinnGR,LingH,WuKD,TsaiTJ,ChenYM,DuffieldJS,LinSL(2013,Jan).Transforminggrowthfactorβstimulatesprofibroticepithelialsignalingtoactivatepericyte-myofibroblasttransitioninobstructivekidneyfibrosis.AmJPathol,182:118-131. ChenYT,ChangFC,WuCF,ChouYH,HsuHL,ChiangWC,ChenYM,WuKD,TsaiTJ,DuffieldJS,LinSL.(2011,Dec).Platelet-derivedgrowthfactorreceptorsignalingactivatespericyte-myofibroblasttransitioninobstructiveandpost-ischemickidneyfibrosis.KidneyInt,80(11):1170-81. LinSL,ChangFC,SchrimpfC,ChenYT,WuCF,WuVC,ChiangWC,KuhnertF,KuoC,ChenYM,WuKD,TsaiTJ,DuffieldJS.(2011,Feb).Targetingendothelium-pericytecrosstalkbyinhibitingVEGFreceptorsignalingattenuateskidneymicrovascularrarefactionandfibrosis.AmJPathol,178(2):911-923. LinSL,LiB,RaoS,YeoEJ,HudsonTE,NowlinBT,PeiH,ChenLJ,ZhengJJ,CarrollTJ,PollardJW,McMahonAP,LangRA,DuffieldJS.(2010,Mar).MacrophageWnt7biscriticalforkidneyrepairandregeneration.ProcNatlAcadSciUSA,107(9):4194-9. HumphreysBD,LinSL,KobayashiA,HudsonTE,NowlinBT,BonventreJV,ValeriusMT,McMahonAP,DuffieldJS(2010,Jan).Fatetracingrevealsthepericyteandnotepithelialoriginofmyofibroblastsinkidneyfibrosis.AmJPathol,176(1):85-97. CastañoAP,LinSL,SurowyT,NowlinBT,TurlapatiSA,PatelT,SinghA,LiS,LupherJr.ML,DuffieldJS(2009,Nov).SerumamyloidPinhibitsfibrosisthroughFcγR-dependentmonocyte-macrophageregulationinvivo.ScienceTranslMed,1,5ra13. LinSL,CastañoAP,NowlinBT,LupherMLJr,DuffieldJS(2009,Nov).BonemarrowLy6Chighmonocytesareselectivelyrecruitedtoinjuredkidneyanddifferentiateintofunctionallydistinctpopulations.JImmunol,183(10):6733-43. LinSL,KisselevaT,BrennerDA,DuffieldJS(2008,Dec).Pericytesandperivascularfibroblastsaretheprimarysourceofcollagenproducingcellsinobstructivefibrosisofthekidney.AmJPathol,173(6):1617-1627. LinSL,ChenYM,ChiangWC,WuKD,TsaiTJ(2008,Sep).EffectofpentoxifyllineinadditiontolosartanonproteinuriaandGFRinCKD:A12-monthrandomizedtrial.AmJKidneyDis,52(3):464-474. ChenYM,LinSL,ChiangWC,WuKDandTsaiTJ (2006,Aug).PentoxifyllineamelioratesproteinuriaviasuppressionofrenalMCP-1insubnephroticprimaryglomerulardiseases.KidneyInt,69(8):1410-1415. LinSL,ChenRH,ChenYM,ChiangWC,LaiCF,WuKD,TsaiTJ(2005,Sep).PentoxifyllineattenuatestubulointerstitialfibrosisbyblockingSmad3/4-activatedtranscriptionandprofibrogeniceffectsofconnectivetissuegrowthfactor.JAmSocNephrol,16,2702-2713. LinSL,ChenYM,ChienCT,ChiangWC,TsaiCC,TsaiTJ(2002,Dec).Pentoxifyllineattenuatedtherenaldiseaseprogressioninratswithremnantkidney.JAmSocNephrol,13:2916-2929. 實驗室網頁  https://sites.google.com/g.ntu.edu.tw/linlabntu/home EnglishIntro Interests Cross-talkbetweenpericytesandendothelialcells/epithelialcellsinprogressiveorganfibrosis Cross-talkbetweenmesohtelialcellsandsubmesothelialfibroblastsinperitonealinjury Multi-potentstemcellsandstromacellsintissuerepair,focusonkidneyandperitoneum Inflammatorymacrophagesinorganfibrosis Trophicmacrophagesintissuerepairandregeneration Pathogenesisofcardiovasculardiseaseinchronickidneydisease,focusonangiopoietin/Tie-2systemandtrophicmacrophages Erythropoietinproducingcellsinnormalandfibrosingkidneys LaboratoryTechniques Geneticallymodifiedmice:Cre-loxPsystem,transgenicmice Animalmodels:organfibrosis(kidney,liver,peritoneum),glomerulonephritis,peritonitis,acutekidneyinjury,bonemarrowtransplantation Virus-deliveredtargeting:adenovirus,retrovirus,lentivirus Primarycellcultures:kidneycells,bonemarrowderivedmacrophages Molecularbiology:RNA,DNA,protein Tissuehistopathology:immunohistochemistry,immunofluorescence Publicationlist ChenYT,HsuH,LinCC,PanSY,LiuSY,WuCF,TsaiPZ,LiaoCT,ChengHT,ChiangWC,ChenYM,ChuTS,LinSL(2020,Jan).InflammatorymacrophagesswitchtoCCL17-expressingphenotypeandpromoteperitonealfibrosis.JPathol,250(1):55-66. ChiangWC,HuangYC,FuTI,ChenPM,ChangFC,LaiCF,WuVC,LinSL,ChenYM(2019,Feb).Angiopoietin1influencesischemicreperfusionrenalinjuryviamodulatingendotheliumsurvivalandregeneration.MolMed,25(1):5. ShihHM,WuCJ,LinSL(2018,Nov).Physiologyandpathophysiologyofrenalerythropoietin-producingcells.JFormosMedAssoc,117(11):955-963. LinYF,LinSL,HuangTM,YangSY,LaiTS,ChenL,WuVC,ChuTS,WuKD;NationalTaiwanUniversityHospitalStudyGrouponAcuteRenalFailure(NSARF)(2018,Oct).New-onsetdiabetesafteracutekidneyinjuryrequiringdialysis.DiabetesCare,41(10):2105-2110. ShihYC,ChenCL,ZhangY,MellorRL,KanterEM,FangY,WangHC,HungCT,NongJY,ChenHJ,LeeTH,TsengYS,ChenCN,WuCC,LinSL,YamadaKA,NerbonneJM,YangKC(2018,Apr).EndoplasmicreticulumproteinTXNDC5augmentsmyocardialfibrosisbyfacilitatingextracellularmatrixproteinfoldingandredox-sensitivecardiacfibroblastactivation.CircRes,122(8):1062-1068. ChouYH,HuangTM,PanSY,ChangCH,LaiCF,WuVC,WuMS,WuKD,ChuTS,LinSL(2017,Apr).Renin-angiotensinsysteminhibitorisassociatedwithlowerriskofensuingchronickidneydiseaseafterfunctionalrecoveryfromacutekidneyinjury.SciRep7:46518. PanSY,ChiangWC,ChenPM,LiuHH,ChouYH,LaiTS,LaiCF,ChiuYL,LinWY,ChenYM,ChuTS,LinSL(2017,Mar).restricteduseoferythropoiesis-stimulatingagentissafeandassociatedwithdeferreddialysisinitiationinstage5chronickidneydisease.SciRep7:44013 ChengSY,ChouYH,LiaoFL,LinCC,ChangFC,LiuCH,HuangTM,LaiCF,LinYF,WuVC,ChuTS,WuMS,LinSL.(2016,Sep).Losartanreducesensuingchronickidneydiseaseandmortalityafteracutekidneyinjury.SciRep6:34265. ChangYT,YangCC,PanSY,ChouYH,ChangFC,LaiCF,TsaiMH,HsuHL,LinCH,ChiangWC,WuMS,ChuTS,ChenYM,LinSL(2016,Feb).DNAmethyltransferaseinhibitionrestoreserythropoietinproductioninfibroticmurinekidneys.JClinInvest,126(2):721-731. ChenYT,ChangYT,PanSY,ChouYH,ChangFC,YehPY,LiuYH,ChiangWC,ChenYM,WuKD,TsaiTJ,DuffieldJS,LinSL(2014,Dec).Lineagetracingrevealsdistinctivefatesformesothelialcellsandsubmesothelialfibroblastsduringperitonealinjuryinmice.JAmSocNephrol,25:2847–2858. ChangFC,ChiangWC,TsaiMH,ChouYH,PanSY,ChangYT,YehPY,ChenYT,ChiangCK,ChenYM,ChuTS,WuKD,LinSL(2014,Jun).Angiopoietin2InducedArterialStiffnessinChronicKidneyDisease.JAmSocNephrol,25:1198-1209. RenS,JohnsonB,KidaY,IpC,DavidsonKC,LinSL,KobayashiA,LangRA,HadjantonakisAK,MoonRT,DuffieldJS(2013,Jan).LRP-6isco-receptorformultiplefibrogenicsignalingpathwaysinpericytesandmyofibroblaststhatareinhibitedbyDKK-1.ProcNatlAcadSciUSA,110:1440-1445. WuCF,ChiangWC,LaiCF,ChangFC,ChenYT,ChouYH,WuTH,LinnGR,LingH,WuKD,TsaiTJ,ChenYM,DuffieldJS,LinSL(2013,Jan).Transforminggrowthfactorβstimulatesprofibroticepithelialsignalingtoactivatepericyte-myofibroblasttransitioninobstructivekidneyfibrosis.AmJPathol,182:118-131. ChenYT,ChangFC,WuCF,ChouYH,HsuHL,ChiangWC,ChenYM,WuKD,TsaiTJ,DuffieldJS,LinSL.(2011,Dec).Platelet-derivedgrowthfactorreceptorsignalingactivatespericyte-myofibroblasttransitioninobstructiveandpost-ischemickidneyfibrosis.KidneyInt,80(11):1170-81. LinSL,ChangFC,SchrimpfC,ChenYT,WuCF,WuVC,ChiangWC,KuhnertF,KuoC,ChenYM,WuKD,TsaiTJ,DuffieldJS.(2011,Feb).Targetingendothelium-pericytecrosstalkbyinhibitingVEGFreceptorsignalingattenuateskidneymicrovascularrarefactionandfibrosis.AmJPathol,178(2):911-923. LinSL,LiB,RaoS,YeoEJ,HudsonTE,NowlinBT,PeiH,ChenLJ,ZhengJJ,CarrollTJ,PollardJW,McMahonAP,LangRA,DuffieldJS.(2010,Mar).MacrophageWnt7biscriticalforkidneyrepairandregeneration.ProcNatlAcadSciUSA,107(9):4194-9. HumphreysBD,LinSL,KobayashiA,HudsonTE,NowlinBT,BonventreJV,ValeriusMT,McMahonAP,DuffieldJS(2010,Jan).Fatetracingrevealsthepericyteandnotepithelialoriginofmyofibroblastsinkidneyfibrosis.AmJPathol,176(1):85-97. CastañoAP,LinSL,SurowyT,NowlinBT,TurlapatiSA,PatelT,SinghA,LiS,LupherJr.ML,DuffieldJS(2009,Nov).SerumamyloidPinhibitsfibrosisthroughFcγR-dependentmonocyte-macrophageregulationinvivo.ScienceTranslMed,1,5ra13. LinSL,CastañoAP,NowlinBT,LupherMLJr,DuffieldJS(2009,Nov).BonemarrowLy6Chighmonocytesareselectivelyrecruitedtoinjuredkidneyanddifferentiateintofunctionallydistinctpopulations.JImmunol,183(10):6733-43. LinSL,KisselevaT,BrennerDA,DuffieldJS(2008,Dec).Pericytesandperivascularfibroblastsaretheprimarysourceofcollagenproducingcellsinobstructivefibrosisofthekidney.AmJPathol,173(6):1617-1627. LinSL,ChenYM,ChiangWC,WuKD,TsaiTJ(2008,Sep).EffectofpentoxifyllineinadditiontolosartanonproteinuriaandGFRinCKD:A12-monthrandomizedtrial.AmJKidneyDis,52(3):464-474. ChenYM,LinSL,ChiangWC,WuKDandTsaiTJ (2006,Aug).PentoxifyllineamelioratesproteinuriaviasuppressionofrenalMCP-1insubnephroticprimaryglomerulardiseases.KidneyInt,69(8):1410-1415. LinSL,ChenRH,ChenYM,ChiangWC,LaiCF,WuKD,TsaiTJ(2005,Sep).PentoxifyllineattenuatestubulointerstitialfibrosisbyblockingSmad3/4-activatedtranscriptionandprofibrogeniceffectsofconnectivetissuegrowthfactor.JAmSocNephrol,16,2702-2713. LinSL,ChenYM,ChienCT,ChiangWC,TsaiCC,TsaiTJ(2002,Dec).Pentoxifyllineattenuatedtherenaldiseaseprogressioninratswithremnantkidney.JAmSocNephrol,13:2916-2929. 實驗室成員 博士班學生 吳岱蓉 施宏謀 鄭鈞耀 駱諳潔 碩士班學生 黃楷婷 楊晶淳 劉倢妤 博士後研究員 曹筱玫 研究助理 吳家齊 邵鈺涵 蔡佩蓁 劉家豪 研究助理：劉家豪、蔡佩蓁、邵鈺涵、吳家齊、陳昱臻 博士後研究員：曹筱玫 主治醫師：張芳綺、陳怡婷、周鈺翔、潘思宇 實驗室畢業生或結訓研究員： 葉沛縈(碩士2013/7,臨床護理師) 張芳綺(博士2014/6,台大醫院主治醫師) 張于庭(碩士2014/7,JohnsHopkinsPhD) 楊千慧(碩士2014/7,台大醫院臨床醫檢師) 廖芳翎(碩士2015/06,義大醫院研究助理) 陳怡婷(博士2016/06,台大醫院主治醫師) 鄭舜陽(碩士2020/09,USCPhDprogram) 劉家豪(碩士2016/06,本實驗室研究助理) 蔡佩蓁(碩士2017/02,本實驗室研究助理) 林紀均(碩士2017/02,藥廠學術專員) 周鈺翔(博士2017/05,台大醫院主治醫師) 邵鈺涵(碩士2018/01,本實驗室研究助理) 潘思宇(博士2018/07,亞東醫院主治醫師) 徐  顥(碩士2018/07,生技公司) 蘇彥昌(碩士2018/07,國防部) 林冠廷(2018,USFPhDprogram) 楊靜欽(博士後研究員,生技公司研究員2018/11) 葉宛儒(博士後研究員,臺師大助理教授2020/08) 魏詩瑤(博士後研究員,哈爾濱醫科大學第二附屬醫院主治醫師2019/08) 陳昱臻(碩士2021/2,本實驗室研究助理) 專任師資 Faculty 招生資訊 Admission 線上捐款 Donation 首頁 成員簡介 生理所主任