Glacios Cryo TEM | Thermo Fisher Scientific - TW

The optimal thermal and mechanical stability of the Glacios Cryo-TEM ensures perfect optical performance. The instrument features a self-assessment function ... 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Home›ElectronMicroscopy›ElectronMicroscopes›TransmissionElectronMicroscopes›GlaciosCryo-TEMTransmissionElectronMicroscopesGlaciosCryo-TEMSampleoptimizationandhighresolutiondataacquisitionmadeeasy. ContactusKeyFeaturesSpecificationsResourcesSoftwareAccessoriesDocumentsContactusPoweredbyBiozTheThermoScientificGlaciosCryo-TransmissionElectronMicroscope(Cryo-TEM)deliversacompletecryo-electronmicroscopy(cryo-EM)solutiontoabroadrangeofscientists.Itfeatures200kVXFEGoptics,theindustry-leadingAutoloader(cryogenicsamplemanipulationrobot),andthesameinnovativeautomationforeaseofuseasontheThermoScientificKrios G4Cryo-TEM.TheGlaciosCryo-TEMbundlesallthisintoasmallerfootprintthatsimplifiesinstallation.Thedesigned-inconnectivityensuresarobustandcontamination-freepathwaythroughouttheentireworkflow,fromsamplepreparationandoptimizationtoimageacquisitionanddataprocessing.TheGlaciosCryo-TEMcanbeusedinasingleparticleanalysisworkflowforpre-optimizationofsamplequality,aswellasforhigh-resolutionsingleparticleanalysisdataacquisition.Furthermore,theGlaciosCryo-TEMcanalsobeconfiguredwithadirectelectrondetectorand/orVoltaphaseplatetobeacompletestandalonesingleparticleanalysisdataacquisitionsolution.PairingtheGlaciosCryo-TEMwiththeThermoScientificSelectrisandSelectrisXImagingFilterscanbeusedasacompletesolutionforsingleparticleanalysisdataacquisitionandcryo-electrontomography(cryo-ET).Overall,theGlaciosCryo-TEMisaversatilemicroscopecoveringallrangesofapplicationssuchassingleparticleanalysis,cryo-ET,aswellmicrocrystalelectrondiffraction(MicroED).Structureofhumanhemoglobin,64.5kDareconstructedwithdatacollectedwithin20hoursontheGlaciosCryo-TEMwithSelectrisXImagingFilterresolvedat3.4Å.UnravellingcomplexproteinstructureswithGlaciosCryo-TEMGABAAReceptor(210kDa)Acquisitiontime:~12hours2.4ÅresolutionGLP1Receptor(150kDa)Acquisitiontime:~20hours2.6ÅresolutionApoferritin(450kDa)Acquisitiontime:~8hours1.6ÅresolutionCDK-cyclin(85kDa)Acquisitiontime:~20hours2.4ÅresolutionStyleSheetforKomodoTabsStyleSheettoremoveellipsisonKomodoTabsstylesheetKeyFeaturesSmallfootprintThenewhardwarearchitectureoftheGlaciosCryo-TEMhasbeenspecificallydesignedtoaddresstheneedforasmallerfootprintandeasieraccesspath.Inmanycases,thisdesignavoidstheadditionalinvestmentandunwantedlabdowntimeforsignificantmodificationstoanexistinginfrastructureortheneedforapurpose-builtlab.EnhancedeaseofusethroughautomationTheGlaciosCryo-TEMreducesthenumberofcomplexalignmentsthatauserhastoperform.Forrecurringalignmentsthatneedtobedoneregularly,automatedalignmentroutineshavebeenimplementedintoThermoScientificEPUSoftwareforsingleparticleanalysis.Furthermore,theEPUuserinterfacehasbeenfurtherstreamlinedtoprovidecomprehensiveuserguidance.MaximumthroughputOptionalEPUMultigridSoftwareallowsdatacollectionforupto12gridstobecompletedovernightwithoutuserattendancebyqueuingmultipleautomaticEPUsessionsfordifferentgridsintheAutoloader.WithThermoScientificEPUDataManagementSoftwarepoweredbyThermoScientificAthenaSoftware,userscanautomaticallysortthecamera’soutput,allowingforeasyorganizationoflargedatasetsandstreamlinedinterfacingwiththeimageprocessingsystem.OptionalEPUQualityMonitorSoftwareperformson-the-flypreprocessingformotioncorrectionandCTFestimation,includingderivedparameters,duringtheactualacquisitionprocess,souserscanjudgethequalityoftheacquireddataandoptimizethedataacquisitionwhileitishappening.ThecombinationofEPU,EPUQualityMonitor,andDataManagementSoftwarehelpsusersgeneratehigh-qualityresultsquickly,confidently,andwiththesameeaseofusethatEPUisknownfor. Reproducible,optimaltoolperformanceTheoptimalthermalandmechanicalstabilityoftheGlaciosCryo-TEMensuresperfectopticalperformance.Theinstrumentfeaturesaself-assessmentfunctionthatautomaticallyevaluatestheopticalstatusofthemicroscope,providingfeedbackforanystepsthatrequireoptimization.Additionally,automatedalignmentroutinesallowtheinstrumenttobetunedtoitsoptimalstartingpointforsingleparticleanalysisortomographyexperiments.WorkflowconnectivityForsuccessfulcryo-EMdataacquisition,optimizationofbothbiochemistryandvitrificationrequiresanefficientoptimizationprocess.TheGlaciosCryo-TEMisdesignedtobeintegratedinasingleparticleanalysisworkflow—itcanbeusedforsampleevaluationandoptimizationbeforehigh-resolutiondataacquisition.ThedataacquisitioncanoccurdirectlyontheGlaciosCryo-TEM,KriosCryo-TEM,oraThermoScientificArcticaCryo-TEMaftersampletransfer.TheAutoloadercapsuleandcassettesystemsensurearobustandcontamination-freetransferofsamplesbetweenGlacios,Arcita,andKriosCryo-TEMswithouttheneedformanipulationofindividualsmallgrids.SpecificationsSourceHigh-brightnessfieldemissiongun(X-FEG)Acceleratingvoltage80–200kVCryo-AutoloaderAutomatedandcontamination-free loadingofcassettes,containingupto12 AutogridsSoftwareEPUSoftwareforSPAsampleassessment,optimization,anddataacquisitionTemperaturemanagementsoftware—includesliquidnitrogenautofillandcooldownschedulingLow-dosesoftwaresuiteminimizeselectrondoseduringcryo-TEMoperationLensesAutomaticcondenser,objectiveandSAaperturesSymmetricconstantpowerobjectivelens(minimizeimageaberrationsandlenshysteresisduringmodeswitching)StagesComputerized4-axesspecimenstagewith±70°alphatiltCryo-stagewithsingleaxisholderImagingRotation-freeimaginguponmagnificationchangesPolepiecegap11mmStructuralcomponentsTwo24”monitorstobeplacedwithin10mofthecolumnCamerasDigitalFluCam –Allmanualandautomaticalignmentscanbe executedwiththesearchandviewcameraCeta 16M CameraCeta-D16MpixelCMOScameraInstallationrequirementhighlightsEnvironmentaltemperature:18°C–23°CDoorheight:2.30m(optionallyreducedto1.96m)Doorwidth:0.90mCeilingheight:2.80mDetectors(optional)Falcon4iDirectElectronDetectorHAADFSTEMdetectorOn-axisBF/DFdetectorsFiltersThermoScientificSelectrisEnergyFilterSoftware(optional)STEMandTEMtomographysoftwareEPUMultigridEPUQualityMonitorforon-the-flypreprocessingServicepackageAccelerateintegratedserviceandapplicationsupportpackagesOtheroptionsVoltaphaseplate OperatingsystemWindows10ResourcesPoweredbyBiozSeemoredetailsonBiozRefineyourpublicationssearchSoftwareForthedifferenttechniquesperformedontheGlaciosCryo-TEM,wehavedevelopedsoftwaresolutionstohelpyougetthemostoutofyourinstrumentation.EPUSoftwareThermoScientificEPUSoftwareisanembeddedsoftwareforsingleparticleacquisition.Itssimplifieduserinterface,advancedautomation,andscreeningcapabilitiesallowforstraightforwardplanningandexecutionofexperiments.Tomography5SoftwareThermoScientificTomography5Softwaredeliversthelatestadvancesincryo-tomography,allowingyoutoacquirevaluable3Ddataevenonyourmostchallengingvitrifiedsamples.EPU-DSoftwareThermoScientificEPU-DSoftwareiselectrondiffractionacquisitionsoftware.Itenablesquickswitchingbetweenimaginganddiffraction,guidesoptimallow-dosesetup,anddrivesthecontinuoustiltfordatacollection.AccessoriesSelectrisandSelectrisXImagingFiltersDesignedforstabilityandspeed,ThermoScientificSelectrisandSelectrisXImagingFiltersarepost-columnimagingfiltersthatimprovethecontrastofTEMimages,resultinginhigh-resolutionstructuresuptoatomicresolution.PairedwiththelatestgenerationFalcon4iDirectElectronDetector,SelectrisFiltersenableyoutoobtainhigh-resolutionstructuresquickly,revealingnewbiologicalinsights.Tofurtherincreasetheproductivityandeaseofuseofyourcryo-EMresearch,thesefiltersarefullyintegratedintoThermoScientificEPUSoftware,ThermoScientificTomographySoftware,andinstrumentoperationsoftware.Falcon4iDirectElectronDetectorTheThermoScientificFalcon4iDirectElectronDetector,withopticalandthroughputenhancementmodesincombinationwithamorestablestage,acceleratesautomateddataacquisition.Aberration-freeimageshift(AFIS)functionalityusesimagebeamshifts,eliminatingtheneedtoperformmechanicalstagemovements.Fringe-freeimaging(FFI)ensuresthebeamisonlycollectingdataonusablepartsofthesample,improvingthequalityoftheresults.WhenconfiguredwiththeSelectrisXImagingFilter,thecapabilitytouseaverystablenarrowslitwidth(<10eV)providesanadditionalboostincontrastforsingleparticlecryo-EMandcryo-ET,improvingresolutionandobtainingresultsfasterwithlessdatatomaximizeyourresolutionefforts.PairedwiththeGlaciosCryoTEMtheFalcon4iDirectElectronDetectormakesitpossibletoefficientlysolvestructuresroutinelyat3.0Åresolutionsorless.Ceta-DCameraTheThermoScientificCeta-DCameraisascintillator-basedcameraoptimizedforlow-dosediffractiondatacollection.Itisidealforworkingwithdose-sensitivematerials,suchasproteins,pharmaceuticalmolecules,andorganicmaterials.Thecamera’shighsensitivityallowsforreliabledetectionofhigh-resolution,low-intensitydiffractionpeaks.Itshighsignal-to-noiseratioallowsforaccuratemeasurementoftheintegratedpeakintensity.Bothoftheseelementsareprerequisitesforobtaininghigh-resolutionstructuralinformation.VitrobotMarkIVSystemTheThermoScientificVitrobotMarkIVSystemcompletelyautomatesthevitrificationprocesstoprovidefast,easy,reproduciblesamplepreparation—thefirststepinobtaininghigh-qualityimagesandrepeatableexperimentalresults.DocumentsDatasheet:GlaciosCryo-TEMeBook:Cryo-electronmicroscopyDatasheet:Cryo-TEMSamplePreparationGuidanceDatasheet:Guidanceforplanningacryo-TEMlaboratoryDatasheet:Cryo-TEMaccessoryoverviewBrochure:SelectedPublicationsofCryo-Tomographywith200kVThermoScientificTalosGlaciosStyleSheettochangeApplicationsH3topwithem-h3-headerclassStyleSheettochangeH3topwithem-h3-headerclassStyleSheetforGlobalDesignSystemStyleSheettochangeH2styletopwithem-h2-headerclassStyleSheetforInstrumentCardsOriginalContactusElectronmicroscopyservicesforthelifesciencesToensureoptimalsystemperformance,weprovideyouaccesstoaworld-classnetworkoffieldserviceexperts,technicalsupport,andcertifiedspareparts.AccelerateandAdvanceforsingleparticleanalysis›AccelerateandAdvanceforcryo-electrontomography›AccelerateandAdvancefordrugdiscovery›Lifescienceserviceandsupport›ElectronmicroscopysupportandresourcesAcceleratingMicroscopyblog›Softwareupdates›Servicerequest›Servicecontracts›Partsinquiry›StyleSheetforSupportandServicefooterStyleSheetforFontsStyleSheetforCards 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